Krameria lappacea (Rhatany or Ratanhia, Krameriaceae) is an endangered, overexploited medicinal plant from the semi-deserts of Andean. Description and images of Krameria lappacea (), a native Chilean plant, provided by the supplier of native exotic Chilean seeds and organizer of plant watching. Krameria lappacea (Rhatany or Ratanhia, Krameriaceae) is an endangered, overexploited medicinal plant from the semi-deserts of Andean South America, and.

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Following parameters were applied: Selected species [ edit ] Krameria argentea Mart. In other projects Wikimedia Commons Wikispecies. Besides gravimetrical analysis of the ratanhiaphenols I, II and III, the content of these compounds in the herbal drug has never been determined. Conclusions In a previous study we could show that not only tannins with their astringent and antimicrobial properties, but also the neglected compound class of lignan derivatives with their pronounced anti-inflammatory activities play an important role in the use of the herbal drug against inflammatory disorders of mouth and throat.

Compound 7 showed highest inter-day variance with maximum deviations of 2.

Krameria lappacea Images – Useful Tropical Plants

Germplasm Resources Information Network. In the course of the present work compound 7, one additional prominent peak, was isolated out of the roots of K. Several mixtures thereof J Pharm Biomed Anal.

Compounds 1 — 6and 8 — 12 were isolated and their structures elucidated in a previous study [10]. In order to present the results in a clear form the extracted ion chromatograms EIC were selected for presentation see Fig.

The composition of the mobile phase consisted of 0. Therefore kraneria developed an efficient and according to the Kraameria guidelines validated HPLC method for the quantification of the active constituents in the roots as well as in the ethanolic tincture.

Open in a separate window. Maximum deviations within one day intra-day precision were 4.

Krameria lappacea Images

Separation was achieved on a phenyl-hexyl column material using a solvent gradient consisting of 0. In order to accomplish a satisfactory separation, all parameters had to lappcea carefully assessed. Three root samples RR-1 to RR-3 and four ethanolic tinctures T-1 to T-43 of them were purchased in pharmacies around Austria, were analyzed during this study, the obtained results are illustrated in Table 3.

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Precision of the whole assay was determined on three following days see Table 2 A and B. Method development The major problem in the development of a HPLC method for the simultaneous detection of all active lignan derivatives was the structural similarity of the analytes.

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Precision of the method was determined by preparing and assaying 5 replicate samples of RR-1 and T-1 on the same day; the same procedure was repeated on two more days. Accuracy was determined in sample RR-1 as well as in sample T-1 T-2 in case of compound 4 by spiking experiments at two different concentrations.

For this purpose 2 methods, liquid—liquid extraction DCM—water and separation over polyamide, were evaluated.

Footnotes Appendix A Supplementary data associated with this article lappacae be found, in the online lapppacea, at doi: Standards Compounds 1 — 6 alppacea, and 8 — 12 were isolated and their structures elucidated in a previous study [10].

One problem emerged at the beginning of sample analysis when evaluating purity of all analytes by the use of the UV purity function of the used HPLC software: In order to check if this extraction protocol is exhaustive, another 5 cycles after the normal extraction protocol were conducted and the obtained extract analyzed by HPLC.

This issue could be explained by the shorter maceration time 3 dayssince regular Tinctura Ratanhiae is prepared by a double maceration over a period of two weeks. Together with the fact that all compounds, except the stereoisomers 2 and 3were baseline separated, selectivity of the assay is indicated. Suitability of the developed method kramerla the quantification of the major lignan derivatives in Ratanhiae radix and Tinctura Ratanhiae can be deduced from several analytical parameters.

The methanol insoluble part Studies on Ratanhiae Radix II.

By comparing variations within the same days intra-day precisions were determined, by observing differences within the 3 days inter-day precision was deduced. Other traditional indications are diarrhoea and mouth ulcer [1]. HPLC sample preparation Extract preparation. Please review our privacy policy.

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The past kkrameria the present uses of Rhatany KrameriaKrameriaceae Econ. This validated assay will be a useful tool for quality control of the plant material and its preparations, focusing on the compound class of lignan derivatives. National Center for Biotechnology InformationU. Proanthocyanidins from Krameria triandra root.

The amount found in the samples was calculated as percent of the initial weight of the dried roots. Highest deviations within the root samples were found for compounds 5 and 12 with contents ranging from 0.

United States Department of Agriculture. The authors thank Ernst P.

The topical anti-inflammatory properties of the secondary metabolites were comparable to the drug indomethacin ID 50 value 0. The content of these compounds in the drug, besides a gravimetrical analysis of the ratanhiaphenols I, II and III content of 0.

Separation was possible by changing the stationary krqmeria from the phenyl-hexyl material to a RP column material, but as highlighted before this made a separation of compounds 11 and 12 impossible.

By mass spectrometry, 1- and 2D-NMR experiments and comparison with published data [7] this lignan derivative was identified as 3-formyl 4-hydroxyphenyl E lappaea. Calibration and validation Individual stock solutions of standard compounds were prepared in methanol. The powdered roots have also served, especially in Portugalto color wines ruby red. Ellmerer for the recording of NMR spectra. Repeatability of the method was assured by relative standard deviations of multiple injections.

For an exhaustive extraction the procedure was carried out twice. Appendix A Supplementary data associated with this article can be found, in the online version, at doi: Wikipedia articles incorporating a citation from the Encyclopaedia Britannica with Wikisource reference Articles with ‘species’ microformats Wikipedia articles incorporating a citation from the Encyclopedia Americana with a Wikisource reference Commons category link from Wikidata.