Adhatoda vasica belonging to Acanthaceae family, commonly known as Adosa, Adhatoda vasica Flowers, Antibacterial activity, Antifungal activity, Diffusion. Abstract. The present study aimed to evaluate the in vitro antimicrobial activity of pharmacologically important Adhatoda vasica plant extracts. Antibacterial activity of leaf extract of Adhatoda vasica was studied against Bacillus subtilis, Antibacterial activity was determined by disc diffusion method.

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Asian Pacific Journal of Tropical Biomedicine. Handbook of Ayurvedic Medicinal Plants. Evaluation of antioxidant activity of isoferulic acid in vitro. Council of Scientific and Industrial Research; Escherichia coli was highly resistant to all the plant extracts at all concentrations. Vasicine acetate could be checked further in drug discovery programme.

Al-Dhabi1 C. Publications and Information Directorate; Minimum inhibitory concentration of Vasicine acetate against tested bacteria and fungi. Antifeedant and toxic activity of Adhatoda vasica leaf adnatoda against Spodoptera littoralis Lep. HPLC chromatograms of the ethanol extract of the leaves of Adhatoda vasica. International Journal of Cancer. The free radical scavenging activity was calculated antibacterrial follows:. The chemicals are given in delta scale with TMS as an internal standard.

It was then distilled on a water bath and the residue was washed with little n-hexane. Vasicine acetate exhibited good zone of inhibition against bacteria: Ray of hope for cancer patients. Potent uterine activity of alkaloid vasicine. Vasicine acetate showed minimum inhibitory concentration values against bacteria; they were M.


Abstract Adhatoda vasica L. Antibacterial activities against Staphylococcus aureus and Micrococcus luteus were observed formethanolic extracts of all the above mentioned plants. Zones of inhibition were recorded in millimetres and the experiment was repeated twice. The physical and spectroscopic data were compared with those reported in the literature [ 2122 ].

Antibacterial activity of adhatoda vasica leaf extract

The free radical scavenging activity was calculated as follows: Antitussive effect of Adhatoda vasica extract on mechanical or chemical stimulation-induced coughing in animals. The fraction was further purified and the compound Vasicine was isolated. Author information Article notes Copyright and License information Disclaimer. HPLC analysis was carried out on a Waters Alliance separations module with photodiode array detector Waters, UV-Visible spectra were taken in methanol on Thermo Fisher instrument.

Kulkarni [ 24 ] reported that A. B revised New Delhi, India: The leaves are used to treat malarial fever, chronic fever, intrinsic hemorrhage, cough, asthma, leprosy, fo diseases, and piles [ 6 ]. The structure of the isolated compound Vasicine and Vasicine acetate Figure 1.

Vasicine acetate showed minimum inhibitory concentration values against bacteria: The solutions were filtered through a membrane filter pore size 0. It is inferred that methanolic crude antibactreial of the above mentioned plantsexhibitantibacterial activities against pathogenic bacteria, which proved the ethnobotanical importance of the selected plants that indigenous people use for cure against various diseases. Preliminary phytochemical and antibacterial screening of crude extract of the leaf of Adhatoda vasica.


The combined chloroform extract was washed with water, dipped, and dried over anhydrous sodium sulphate. Vasicine acetate was obtained by acetylation of Vasicine. Vasicine hydrochloride, a new drug for interruption of pregnancy. DPPH 2,2-diphenylpicrylhydrazyl radical scavenging activity of Vasicine acetate was determined based on the method of Wang et al.

Cytotoxic effect of Vasicine acetate against A lung adenocarcinoma cancer cell line. Structure of the compounds: The alkaloid Vasicine was isolated from ethanolic extract of the leaves of A.

Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Every year, at leastpeople die worldwide from cancer related to their workplace [ 3 ]. Balachandran2 S. The structure was elucidated using spectroscopic methods: International Journal of Green Pharmacy.

All melting point values are uncorrected and taken acrivity open capillary method on a heating block Instrument. Greater antibacterial activity against Pseudomonas aeruginosa was only exhibited by Phyllanthus emblica, Pinus roxburghii, Debregeasia salicifolia and Lantana camara.

Journal of Natural Product and Plant Resources. Acanthaceae is used in the indigenous system of medicine in India. Journal List Biomed Res Int v.